Regulatory

Part:BBa_K112707:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


Pspv from Salmonella


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1285
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Construction of <Pspv> basic part K112707

PCR of dv017/dv016 on pBca1037         (499bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                       (EcoRI/BamHI, 2472+499, L)
Product is pBca1256-K112707
-----------------------------------------------
dv017 Forward biobricking of <Pspv>
cgataGAATTCatgAGATCTaGATCCTGTGATGTTTGGCG

dv016 Reverse biobricking of <Pspv>
cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG

References